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KMID : 0861020170320010075
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Korea Journal of Herbology
2017 Volume.32 No. 1 p.75 ~ p.81
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Antioxidative and Antimicrobial Activities of Herbal Complex Extract from Hwangryunhaedok-tang and Essential Oil of Chamaecyparis obtusa
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Kim Bo-Ae
Park Shin-Ho
Yang Jae-Chan
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Abstract
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Objectives : The present study was designed to investigate effects of mixed extracts (9:1, v/v) of Hwangryunhaedoktang, Chamaecyparis obtusa essential oil. We evaluated the antimicrobial and antioxidant activity by manufacturing mixed extracts as the materials for functional medicinal herb cosmetics.
Methods : We performed antimicrobial were tested microbes (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, Propionibacterium acnes) by disc diffusion method and measure clear zone. Antioxidant activities were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity.
Results : Antimicrobial activities of mixed extracts against S. epidermideis, P. acnes, S. aureus, E. coli, and Candida. A were 10.9±3.0 ㎜, 16.9±2.0 ㎜, 9.2±2.0 ㎜, 10.3±1.0 ㎜, 11.8±3.0 ㎜ respectively. The Chamaecyparis obtusa essential oil had the highest antimicrobial activities against S. epidermideis, P. acnes, S. aureus, E. coli, and Candia. A and clear zone of microbes ware 16.7±3.0 ㎜, 28.8±0.2 ㎜, 15.9±2.0 ㎜, 11.5±1.0 ㎜, 16.3±3.0 ㎜. Hwangryunhaedoktang extract showed antimicrobial activity but only P. acnes and S. aureus The antioxidant activities of the mixed extracts were tested through the evaluation of DPPH radical scavenging activity. The 100 % mixed extracts were found to have 90 % DPPH radical scavenging activity. The mixed extracts was presented similar antioxidant activities compared with that of ascorbic acid. As a result, A mixture extract is expected to have antimicrobial effects and free radical scavenging activity was found.
Conclusions : Accordingly, It can be concluded that mixed extracts has the potential to cosmetic material.
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KEYWORD
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Hwangryunhaedok-tang, Chamaecyparis obtusa essential oil, Antimicrobial activity, Antioxidative activity
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